• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Incubator (6) for biological material (M), with a housing (7), an incubation chamber (K) arranged in the housing (7) for a biological material (M), a viewing device (8) arranged in the housing (7) for observation of the biological material (M) in the incubation chamber (K), the viewing device (8) having at least one viewing window (9) and one viewing channel (10), and a first temperature control device (11) for the viewing device (8), the first Temperature control device (T1) a first temperature control chamber (12) surrounding the viewing channel (10), a first inlet opening (13) through which a temperature control fluid (T) can flow into the first temperature control chamber (12), and a first outlet opening (14) which the temperature control fluid (T) can flow from the first temperature control chamber (12).
    公开号:AT522439A4
    申请号:T50349/2019
    申请日:2019-04-17
    公开日:2020-11-15
    发明作者:
    申请人:Hektros S R L；
    IPC主号:
    专利说明:

    The present invention relates to an incubator for biological material, with a housing, an incubation chamber for a biological material arranged in the housing, a viewing device arranged in the housing for observing the biological material in the incubation chamber, the viewing device having at least one viewing window and one viewing channel, and a first
    Temperature control device for the viewing device.
    Incubators (often called cultivation devices) are used to study small organisms and their development through a microscope.
    Such incubators are mostly used in biotechnology laboratories.
    A general example of an incubator can be found in AT 500 473 B1. Here
    it is specifically about openings for the introduction of manipulation instruments.
    A generic document is DE 102 59 251 B4, which describes a cultivation chamber that can be attached to a microscope stage or a microscope table. A lid of this cultivation chamber is made of Teflon and the window attached to the lid consists of two layers of heat-conducting glass. The window is heated to keep the cultivation chamber warm and to prevent the window from misting up. Specifically, an annular heating resistor is inserted between the two layers of glass to provide heat and prevent the glass from fogging up. The disadvantage here is that the annular heating resistor is very expensive in terms of production technology and costs. Above all, this heating resistor must be supplied with electricity by means of its own electrical line. In addition, a precise feedback control and coordination with the temperature in the cultivation chamber is difficult or expensive, that is to say can hardly be achieved without the formation of uncontrollable temperature gradients. In addition, an external, likewise heated air humidification device is required, into which the incubation gas is first introduced before it is fed to the incubation chamber. Depending on the ambient temperature on the microscope, this creates the problem of water vapor undersaturation
    and condensation in the supply channel to the cultivation chamber. In addition, the Chamber must
    which makes handling difficult.
    The object of the present invention is therefore to create a simple and less complex incubator. In particular, the status of
    Technology given disadvantages to be remedied.
    This is achieved by an incubator with the features of claim 1. Accordingly, the invention provides that the first temperature control device has a first temperature control chamber surrounding the viewing channel, a first inlet opening through which a temperature control fluid can flow into the first temperature control chamber, and a first outlet opening via which the temperature control fluid can flow out of the first temperature control chamber. This means that no separate power supply is necessary, but a temperature control fluid from a circulation thermostat, as is common in most laboratories, is used to prevent fogging. As a result, the viewing window does not fog up and it is an observation of the biological
    Material always possible through the viewing window and the viewing channel.
    Preferred embodiments of the present invention are shown in
    dependent claims.
    The shape of the housing is arbitrary. For example, the housing can be designed in the shape of a cuboid. For simple handling, however, it is preferably provided that the housing is essentially cylindrical and has a central axis, this central axis leading through the viewing channel, the at least one viewing window and the incubation chamber. Thus are the
    Viewing channel and viewing window arranged exactly in the middle of the housing. It is in itself possible for two or more viewing devices to be formed in the housing. However, it is preferably provided that only one in the housing
    Viewing device and only one incubation chamber are formed.
    In order to enable the biological material to develop well, is preferred
    one formed in the housing and the incubation chamber in certain areas
    can flow into the incubation chamber.
    For the development of the biological material in the incubation chamber, it is also essential that the temperature in the incubation chamber is as constant as possible. For this reason, a second temperature control device (separate from the first temperature control device) is preferably provided for the incubation chamber, the second temperature control device over a second temperature control chamber surrounding the incubation chamber, a second inlet opening through which a temperature control fluid can flow into the second temperature control chamber, and a second outlet opening the temperature control fluid from the second
    Can flow temperature chamber, has.
    The exact geometric configuration of the viewing channel and / or the incubation chamber is arbitrary per se. For example, these components can be formed in or through a cuboid wall. However, it is preferably provided that the viewing channel and / or the incubation chamber are / is delimited at least in some areas by a wall which is in the shape of a circular cylinder or a cone, preferably formed around the central axis. The circular cylinder jacket-shaped wall of the viewing channel and the circular cylinder jacket-shaped wall of the
    Incubation chamber on the same radius.
    It is possible that the first and / or second temperature control chamber are / is only formed in areas in the housing or only adjoin the viewing channel and / or the incubation chamber in areas. For the most homogeneous possible introduction of the temperature control power, however, it is preferably provided that the first and / or second temperature control chambers are annular around the central axis
    are / is. Furthermore, it is preferably provided that the first and / or second temperature control chamber
    an inner wall surface, an outer wall surface, a floor surface, and
    are / is limited to a top surface. The inner wall surfaces are particularly preferred
    or central axis are formed and run parallel to one another.
    According to a preferred embodiment it is provided that in the first and / or second temperature control chamber separate guide elements from the two wall surfaces for the flow line of the inflowing temperature control fluid are arranged. These guide elements serve, on the one hand, to homogenize the temperature control fluid and, on the other hand, to ensure that the warm temperature control fluid that has just been introduced is directed inwards to the viewing channel or to the incubation chamber. In the first temperature control device, the guide elements serve primarily to prevent the viewing window from fogging up. In the second temperature control device, the guide elements serve to ensure that the introduced, still warm temperature control fluid is passed directly to the conversion of the incubation chamber in which the biological
    Material is located so that the desired temperature is there.
    The guide elements can only partially or partially protrude into the temperature control chamber. For a good guiding effect, however, it is preferably provided that the guiding elements are designed as guiding walls which are connected to the bottom surface and / or to the top surface. Specifically, the guide walls of the first temperature control device are designed in one piece with the top surface and the guide walls of the second temperature control device are designed in one piece with the bottom surface
    educated.
    In order to achieve a targeted flow in the temperature control chamber, it is preferably provided that the guide elements are spaced apart from the wall surfaces and that there are throughflow openings between the guide elements and the wall surfaces
    are trained.
    Furthermore, it is preferably provided that the guide elements are arranged at regular intervals from one another and equally spaced from a central axis. There can preferably be three, four, five or six guide elements in the temperature control chamber
    be provided.
    formed, which redirects the flow accordingly.
    Furthermore, it is preferably provided that the guide elements comprise several, preferably five, side bars, the side bars being arranged separately and at a distance from the main bars in the temperature control chamber. The side webs are preferably less than 5 mm, preferably between 0.5 mm and 2 mm, from the
    outer wall surface spaced.
    As an alternative to the guide elements, it can be provided that in the first and / or second temperature control chamber a meander-shaped one delimited by guide walls
    Tempering is formed.
    A particularly homogeneous and advantageous temperature control is achieved in that the temperature control channel has an essentially radially extending inflow section, a first ring section adjoining the inflow section, bounded directly by the inner wall surface and leading around the central axis, and several first ring sections radially outward to the first ring section comprises subsequent ring sections which follow one another in a meandering shape and lead around the central axis. As a result, the temperature control fluid is first directed into the inner (to the viewing channel or to the incubation chamber) and ensures the appropriate temperature control there. Then the temperature control fluid is ring section for
    Ring section directed to the outside and gradually cools down slightly. A second aspect of the present invention relates to an incubator for
    biological material, with a multi-part housing, one of the housing
    formed incubation chamber for a biological material, at least one of the
    can.
    Incubators known to date, such as those which emerge from AT 500 473 B1 and DE 102 59 251 B4, are intended for frequent use. As a result, they are built quite elaborately and cost a lot. In addition, a
    extensive cleaning can be carried out after use.
    The object of the second aspect of the invention is therefore to create a less complex and simple incubator which nevertheless provides all the basic functions. Most of all, the incubator is designed for one
    one-time (and pre-sterilized) use.
    This is achieved by an incubator having the features of claim 16. Accordingly, it is provided according to the invention that the housing consists entirely of at least partially transparent, preferably injection-molded, plastic. In other words, the incubator consists of plastic that is transparent at least in some areas. As a result, all the basic functions are available, but the individual components are made in a simple way from a plastic and not from metal or other complex materials. The incubator can therefore be manufactured and sold relatively cheaply, so that
    this is ideal for a single use.
    7747
    the housing comprises exactly five, each one-piece components.
    Specifically, a base element is provided as the first component, in which part of the incubation chamber, the second inlet opening, the second outlet opening, the gas supply opening and part of the second temperature control chamber are formed. A first intermediate cover which can be connected to the base element, preferably frictionally, is provided as the second component, which together with the base element forms the second temperature control chamber. A perforated cover element arranged on the first intermediate lid is provided as a third component, which, together with the intermediate lid, forms the humidification chamber. The fourth component is a cover element which can be connected to the base element, preferably frictionally, in which the viewing device, the first inlet opening, the first outlet opening and part of the first temperature control chamber are formed. A second intermediate cover which can be connected to the cover element, preferably frictionally, is provided as the fifth component, which together
    forms the first temperature control chamber with the cover element.
    Furthermore, it is preferably provided that a first, preferably planar or lens-shaped, viewing window is formed in the cover element and a second viewing window is formed in the second intermediate cover, the second viewing window delimiting the incubation chamber. The viewing channel is through its
    Wall and the two viewing windows limited or formed.
    It is possible that the individual components consist of different plastics that are best suited for the respective application. For simple production, however, it is preferably provided that the five components consist of
    consist of the same plastic.
    It is particularly preferably provided that the plastic is a transparent one
    Thermoplastic, preferably polystyrene, is.
    Gas pipe.
    It is particularly preferably provided that the temperature control fluid line has a first line section which connects the temperature control fluid source to the first inlet opening, a second line section which connects the first outlet opening to the second inlet opening, and a third line section which connects the second outlet opening to the temperature control fluid source . In this way, the “fresh” temperature control fluid first enters the first temperature control chamber at a slightly higher temperature, where it cools down slightly, and then into the second temperature control chamber. Due to the slightly higher temperature in the first temperature chamber, fogging of the at least one viewing window in the
    prevents damp interior of the incubator.
    The temperature control fluid can be in the form of a temperature control gas or a temperature control liquid. For example, a special mixture of different substances can be used as the temperature control liquid. However, it is preferably provided that water is used as the temperature control fluid. With water as the temperature control fluid, a more homogeneous heat distribution is achieved thanks to its high heat capacity. Water has the added benefit of being quite heavy. Thus, the housing, which is actually light and made of plastic, can be placed well and stably on the microscope due to the additional weight of the water.
    There is a secure hold.
    A nutrient gas is introduced from the gas source into the incubation chamber via a gas line and the gas supply opening. A mixture of 95% air and 5% carbon dioxide, for example, can be used as the nutrient gas. A pH-buffered aqueous solution of sugars, amino acids, vitamins, salts and protective substances against microbial infection can be used as the nutrient medium. A nutrient medium (also known as a culture medium) is used for cultivation
    of biological material. Specifically, microorganisms, cells, tissue
    are located.
    Important points and features of the present invention (s) are set forth in
    the following paragraphs are sometimes given with different words:
    A general problem with incubators for live cell observation is that, due to the system, no heating is possible in the culture container (incubation chamber) arranged in the middle or in the overlying see-through area. It can therefore be colder here, which makes condensation and fogging of the surfaces with water droplets more likely. This problem is solved by the first temperature control device. This is designed above the culture container in a cover part in the form of a correspondingly large, cylindrical compartment. This is welded to the top surface and is also high enough to prevent condensation. Here
    an air cushion forms towards the culture container, which has a (heat) insulating effect.
    The incubator is heated with water (temperature control fluid), with the water first entering the first temperature control chamber at the cover part and only flowing through a bridge (second line section) into the base part after flowing through the cover part. Since a little heat is given off when flowing through the lid, it is slightly warmer in the lid part than in the bottom part. This must be taken into account when setting the temperature for the culture of the cells (biological material), but it makes it possible that the top surface of the cover part does not fog up and thus an observation of the organisms in the culture chamber below is and remains possible.
    This is achieved above all by the fact that it is warmer in the lid area.
    At least in the cover part, but preferably also in the bottom part, devices for guiding the heating medium are provided (guide elements or guide walls). Due to the geometric design of the devices, the centrally arranged culture container is evenly washed around by the heating medium (temperature control fluid) and thus
    also heated evenly.
    There are two possible designs for the flow control:
    In the case of a spiral or meander-shaped channel, the entire temperature chamber is flowed through evenly and thus the entire area is heated. The heating medium can initially flow from the inlet opening directly to the culture container via a radial channel. The spiral-shaped or meander-shaped then begins on the inside and runs outward with increasing radius until the outlet opening is reached. The temperature at the culture container must meet the requirements of the organisms; it should be the warmest here or the setpoint temperature should prevail. A radial temperature loss is
    acceptable and even desired.
    In a second variant, web-shaped baffles (guide elements) are arranged in the temperature control chamber. Here, circular currents arise around the baffles, which ultimately means that the culture container in the form of a central vortex that is as homogeneous as possible is flowed evenly (circular, laminar) around. Accordingly, here is the
    The arrangement and orientation of the harassment is essential.
    An arrangement is particularly favorable in which a spiral-shaped or meander-shaped channel is arranged in the cover and baffles are arranged in the bottom part. Due to the larger contact area of the inner walls, more heat is given off in the bottom part. As a result, the temperature difference in the axial direction is greater. This is
    favorable for avoiding condensation in the central area.
    The circuits for the heating medium (temperature control fluid) are completely separated and
    separately from the supply of the nutrient medium.
    With a culture container diameter of 20 mm there is a temperature difference between the wall of the culture container and the center of the culture container of about 0.8 ° C. With a culture container diameter of 25 mm there is a temperature difference between the culture container wall and the center of the culture container of about 1, 2 ° C. The smaller variant is preferred, since it allows more homogeneous temperature control of the culture chamber. So there is too great a temperature difference
    unfavorable.
    The nutrient gas flows through a gas supply opening into a humidification chamber in which distilled water or a moistened sponge is arranged. The nutrient gas absorbs moisture from the surrounding water or from the sponge and flows through upper openings into the culture container and then at the edge of the lid area first in the direction of the lid surface and then ultimately radially between
    Cover and edge of the incubator.
    The passage opening for the nutrient gas at the bottom of the humidification chamber is selected to be correspondingly small so that no water can flow out here. It is also conceivable that the upper passage openings of the humidification chamber are of different sizes or have a different number in order to allow the flow and
    Control distribution of the nutrient gas.
    The top of the culture container protrudes into the so-called atmospheric cavity in the form of a web. This creates a sterilization edge that is a
    Can avoid contamination of the cell culture with bacteria.
    The sponge is moistened with sterile and deionized water through the upper openings with a pipette or a syringe. The humidification via the
    A sponge through which nutrient gas flows is usually sufficient for at least two days.
    Drip ribs in the edge area of the cover part are also essential. This edge area represents the coldest zone in the incubator. Compared to previous versions, the drip ribs are larger and have a notch on the lower edge. Condensation can collect there and return to
    Drain the humidification chamber.
    In order to avoid condensation in the area of the viewing window, the flow of the heating medium in the cover is most important. A radial temperature loss (towards the outside) is wanted in order to keep the condensation in the outer areas. In short: the heat should be directed to areas where there is no condensation
    should take place.
    Increased condensation in the area around the edge of the incubator means that less humidification of the atmosphere is lost due to the outflowing, moist nutrient gas
    and the incubator can be operated for longer (long incubation time possible).
    Another problem is the heat dissipation to the outside when the incubator is placed on a metal microscope. In order to minimize the contact area, the bottom surface has three concentric bars on which the incubator stands. Thereby
    the direct heat conduction from the incubator to the support is minimized, with additional
    an insulating air cushion is created.
    The incubator is controlled by the temperature of the water and the room temperature. Feedback is possible by inserting a thermometer through an additional channel or bar. In contrast to a high-end device made of metal, where a temperature release to the outside is wanted and is possible and balanced by a very precise temperature control, this is a sluggish system. The control accuracy is still around + 0.5 ° C (with the high-end device + 0.05 ° C).
    A (printed) lens can be placed in the lid or in the culture container for observation
    be included.
    Further details and advantages of the present invention will become apparent from the description of the figures with reference to those shown in the drawings
    Exemplary embodiments are explained in more detail below. Show in it:
    1 schematically shows an arrangement with an incubator,
    2 shows an exploded view of an incubator in perspective,
    3 shows the bottom element and the cover element in perspective,
    Fig. 4 is a perspective view of the base element, the first intermediate cover and the
    Cover element,
    5 shows the cover element and the second intermediate cover in perspective,
    6 the floor element including the first intermediate cover with various cuts,
    7 shows the cover element including the second intermediate cover with various cuts,
    Fig. 8-11 Sections through the temperature chamber with differently designed
    Elements for flow control,
    FIG. 12 shows a vertical section through the incubator with the gas flow illustrated, FIG. 13 shows a plan view of an alternative exemplary embodiment of the
    Cover element and FIG. 14 a perspective view of an alternative embodiment
    of the floor element.
    1 shows an arrangement 40 with an incubator 6 including housing, a temperature control fluid source 41, a temperature control fluid line 42, a gas source 43 and a gas line 44. The temperature control fluid source 41 contains a temperature control fluid T. This temperature control fluid T can for example be water. The temperature control fluid source 41 is connected to the first inlet opening 13 of the first temperature control device 11 via the first line section 42a of the temperature control fluid line 42. The temperature control fluid T reaches the first temperature control chamber 12 via this first inlet opening 13. This temperature control chamber 12 is formed around the viewing device 8 at least in regions. The viewing device 8 has at least one viewing window 9 and one viewing channel 10. Specifically, a first (upper) viewing window 91 and a second (lower) viewing window 92 are provided. The first temperature control device 11 also has a first outlet opening 14, via which the temperature control fluid T can exit again from the temperature control chamber 12. From there, the temperature control fluid T reaches the second inlet opening 23 of the second temperature control device 21 via the second line section 42b. This second temperature control device 21 has a second temperature control chamber 22 which at least partially surrounds an incubation chamber K (can also be referred to as a cultivation chamber). Biological material M is contained in this incubation chamber K. This biological material M can contain, for example, cells, microorganisms or the like. The temperature control fluid T can then leave the incubator 6 again via the second outlet opening 24 of the second temperature control device 21. The brought temperature control fluid can then be disposed of via the third line section 42c or can flow into a drain. However, it is preferably provided that the temperature control fluid T is returned via the third line section 42c
    is passed to the temperature control fluid source 41. Thus there is a temperature control fluid circuit
    given is. The incubator 6 also has a gas supply opening 39. Gas G (e.g. a dry gas mixture consists of 95% air and 5% CO 2) enters the incubator 6 via this gas supply opening 39. The gas G can then escape again through narrow openings or gaps, not shown here. The nutrient medium, which supplies the living biological material M with nutrients, is directly in
    the incubation chamber K introduced.
    Such an arrangement 40 with an incubator 6 is mostly used in laboratories in order to observe the growth and further development of the biological material M via a microscope. For this it is particularly important that unhindered observation of the biological material M is possible via the viewing device 8. Since the temperature control fluid T is usually heated to a range between 30 ° and 50 ° C., preferably to 37 ° to 40 ° C., it is possible that parts of the incubator 6 may fog up. It is particularly disadvantageous if the at least one viewing window 9 fogs up. In order to avoid this, the viewing device 8 is temperature-controlled via the first temperature control device 11. During this temperature control, the temperature control fluid T cools down slightly, so that a slightly lower temperature prevails in the second temperature control chamber 22 (for example 1 ° C. lower). As a result, it is slightly warmer in the (upper) area of the incubator 6, which prevents fogging. The temperature control fluid T thus has a double function: on the one hand, the incubation chamber K is kept at a constant, ideal temperature. On the other hand, the temperature control fluid T is also used
    To prevent fogging of the viewing device 8 or the viewing windows 91 and 92.
    An exploded view of an embodiment of an incubator 6 is shown in perspective in FIG. This incubator 6 has all the essential components of an incubator 6, but is nevertheless quite simply constructed. Above all, this incubator 6 consists of a few individual components, each made of a transparent plastic (preferably injection-molded polystyrene). Specifically, the housing 7 of this incubator 6 is made up of five components: base element 1, first intermediate cover 2, cover element 3, cover element 4 and
    second intermediate cover 5.
    The second intermediate cover 5 can be inserted into the cover element 4. The first temperature control chamber 12 of the first temperature control device 11 is thereby formed by the cover element 4 and the second intermediate cover 5. The first inlet opening 13 and the first outlet opening 14 for the temperature control fluid T can also be seen in FIG. 2. The first temperature control chamber 12 is annular around the central axis Z. The central axis Z leads through the first viewing window 91 and the viewing channel 10 as well as through the second viewing window 92, which is formed by the second intermediate cover 5. The bottom surface 12b of the first temperature control chamber 11 is also formed on the second intermediate cover 5. In contrast, the inner wall surface 12i and the outer wall surface 12a of the first temperature control chamber 11 are formed on the cover element 4. The top surface 12d of the first temperature control chamber 11 is also formed on the cover element 4. The outer circumference of the retaining ring 45 formed on the second intermediate cover 5 corresponds to an inner circumference of a retaining ring 46 formed on the cover element 4. By sliding the second intermediate cover 5 onto the cover element 4, these two components 4 and 5 are frictionally connected to one another via the corresponding retaining rings 45 and 46 held. The second intermediate cover
    5 thus forms a type of cover with which the temperature control chamber 12 is closed.
    The situation is similar with the base element 1 and the first intermediate cover 2. The first intermediate cover 2 has a central opening 47. The wall of the incubation chamber K can be pushed through this. The outer circumferential surface 48 corresponds to the inner circumferential surface 49 of the base element 1. By pushing the first intermediate cover 2 into the base element 1, the two components 1 and 2 are frictionally connected to one another, the outer circumferential surface 48 being pressed or pressed against the inner circumferential surface 49 of the base element 1 is pressed. In this assembled state, the second temperature control chamber 22 is formed between the two components 1 and 2. This second temperature control chamber 22 has the top surface 22d formed by the first intermediate cover 2. In addition, the second temperature control chamber 22 is formed or delimited by the outer wall surface 22a, the bottom surface 22b and the inner wall surface 22i, each formed on the bottom element 1. The second inlet opening 23 and second are also located on the bottom element 1
    Outlet opening 24 for the temperature control fluid T is formed. Furthermore, this points
    The bottom element 1 of the incubator 6 has the gas supply opening 39. In this base element 1, the guide elements 31 in the form of the main webs 33 and the side webs 36 can also be seen. In addition, a gas supply chamber 50 is formed in the base element 1. This is covered by the cover section 51 formed on the first intermediate cover 2. In this cover section 51 there is a passage opening 52. Via this passage opening 52, the gas G supplied via the gas supply opening 39 can flow upwards and reaches an area between the cover element 4 and the base element 1. The base element 1 is in the closed or assembled state connected to the cover element 4, since the inner circumferential surface 53 of the cover element 4 rests against the outer circumferential surface 54 of the base element 1. The cover element 4 is thus pushed onto the base element 1. Precise positioning is ensured by the guide elements 55 formed in the cover element 4 and the corresponding guide recesses 56 formed in the base element 1
    enables.
    In the closed state, the cover element 3 is located between the cover element 4 and the base element 1. This cover element 3 rests on the first intermediate cover 2. A humidification chamber 38 is located between the first intermediate cover 2 and the cover element 3. A highly absorbent polymer sponge can be inserted into this humidification chamber 38. This allows up to 20 ml of water to be absorbed. The cover element 3 has a central opening 57. With this central opening 57, the cover element 3 can be plugged onto the outer wall of the incubation chamber K. In addition, several (specifically twelve) radial gas passage openings 58 are formed in the cover element 3. These have a diameter of about 1 mm. The gas passage openings 58 serve, on the one hand, to fill the humidification chamber 38 with sterile water and, on the other hand, to allow gas to pass through, whereby the gas G entrains the water in the humidification chamber 38 as it flows through the humidification chamber 38, whereby a humidity of about 98% is reached. The water vapor-enriched gas G that has passed through the gas passage openings 58 then passes through the central opening 57 into the incubation chamber K and prevents the biological material M from drying out there.
    In Fig. 3 the components of the incubator 6 are shown partially assembled. The cover element 4 has the first inlet opening 13 and the first outlet opening 14. The base element 1 has the second inlet opening 23, the second outlet opening 24 and the gas supply opening 39. In addition, parts of the second line section 42b and of the third line section 42c are shown. In addition, part of the gas line 44 is also shown. The cover element 3 is received in the base element 1 and has the gas passage openings 58.
    The guide recesses 56 can also be seen.
    In Fig. 4 the base element 1, the first intermediate cover 2 and the cover element 3 are shown. In addition, the second temperature chamber 22 and the humidification chamber 38 are shown as perspective objects. Of course, these are not specific components, but the dimensions and shapes of these two chambers 22 and 38 result from the walls and surfaces of the other components 1, 2 and 3. In any case, it can be seen that both the second temperature chamber 22 and the Humidification chamber 38 are each formed in a ring around the central axis Z. The second temperature chamber 22 is suitable for holding 30 to 50 ml, preferably approximately 40 ml, of temperature control fluid T. The humidification chamber 38 is designed to accommodate 15 to 25,
    preferably of about 20 ml, liquid suitable.
    In the same way, the cover element 4 is shown in perspective in FIG
    second intermediate cover 5 and first temperature control chamber 12 formed between them. This temperature chamber 12 contains 30 to 50 ml, preferably about 40 ml, of temperature control fluid T. Here, too, of course, the inner surfaces or
    Walls of the components 4 and 5, the temperature chamber 12.
    In Fig. 6, matching to Fig. 4, the bottom element 1 and the first intermediate cover 2 are shown at the top right. A plan view of this part of the incubator 6 can be seen in the lower left area. In this plan view, the cutting lines B-B and C-C are drawn. In the section C-C shown at the bottom right, the gas supply opening 39 can be seen, through which gas G enters the gas supply chamber 50
    got. The gas G that has flowed in passes through the passage opening 52
    through the first intermediate cover 2 in the direction of the humidification chamber 38. It can be seen in this section C-C that the first intermediate cover 2 rests in the outer area on an inner wall of the base element 1 and in the inner area rests against the wall of the incubation chamber K on the outside. As a result, the second intermediate cover 2 is connected relatively firmly to the base element 1 and the second temperature chamber 22 is formed between these two components 1 and 2. This connection between the first intermediate cover 2 and the base element 1 can also be seen in section B-B. Section line A-A is also drawn in section B-B. The associated section A-A is shown at the top left of FIG. Above all, the second inlet opening 23, the second outlet opening 24 and the gas supply opening 39 as well as the passage opening 52 result from this section A-A. The guide elements 31 can be seen particularly well in this section A-A. Specifically, there are two types of guide elements 31. On the one hand, these are the main webs 33 and, on the other hand, the side webs 36. The side webs 36 are located relatively close to the outer wall surface 22a of the second temperature control chamber 22 and are approximately 35 ° to 55 ° to the radial direction inclined. The guide elements 31 serve to guide the flow of the inflowing temperature control fluid T. Between the guide elements 31 and the wall surfaces 22i and 22a, throughflow openings 32 are formed. The guide elements 31 are arranged at regular intervals from one another and at a uniform distance from a central axis Z. The main webs 33 comprise a first, radially outer, essentially radially aligned, straight guide section 34. In addition, the main webs 33 have a straight, second guide section 35 that is closer to the central axis Z. The second guide section 35 is at an angle between 15 ° and 65 ° to the first
    Guide section 34 aligned. In this case this angle a is approximately 40 °.
    7 contains the same representations as in FIG. 6, only in relation to the cover element 4 and the second intermediate cover 5. At the top right, the cover element 4 together with the first inlet opening 13 and first outlet opening 14 and the second intermediate cover 5 are shown. A top view of the cover element 4 is shown at the bottom left. In this top view, the cutting lines H-H and II are drawn. In the section I-I shown at the bottom right, the first outlet opening 14 is shown, which branches off from the first temperature control chamber 12. The second
    Intermediate cover 5, in particular its retaining ring 45, corresponds to
    Retaining ring 46 of the cover element 4 is formed. The two viewing windows 91 and 92 and the viewing channel 10 of the viewing device 8 can be seen particularly well from the section H-H. These are arranged centrally in the incubator 6. The drip chambers 59 can also be seen in this section H-H. The section line G-G is drawn in section H-H. The associated section G-G is shown in Fig. 7 top left. In this section G-G, the drip chambers 59 arranged in a ring around the incubation chamber K and the drip ribs 60 can be clearly seen. The incubation air rising from the incubation chamber K is saturated with water vapor and cools in the lid edge area and condenses on the walls of the draining chambers 59 and on the draining ribs 60. The condensed water then drips back into the humidification chamber 38. In section G-G, the inlet opening 13 and the first outlet opening 14 as well as the first temperature control chamber 12 are also shown. The first temperature control chamber 12 is laterally bounded by the outer wall surface 12a and the inner wall surface 12i. Guide elements 31, which are again subdivided into side webs 36 and main webs 33, are also arranged in this first temperature control chamber 12. These main webs 33 also have
    a first guide section 34 and a second guide section 35.
    In the figures 8, 9, 10 and 11 further variants are shown how the temperature chambers 12 and 22 are formed and especially how they are
    Flow line takes place.
    FIG. 8 is essentially identical to the embodiment according to FIG. 6, only the second guide section 35 of the main webs 33 is less strong than the first guide section
    34 angled.
    In contrast, FIG. 9 is essentially identical to the embodiment according to FIG. 7. Here, too, the second guide sections 35 are less pronounced than the first
    Guide section 34 angled.
    In FIG. 10, in line with FIG. 8, the flow of the temperature control fluid T is also shown. The thickness of the arrows indicates the strength and / or speed of the flow. In general, the baffles (guide elements 31) are designed so that the central
    Incubation chamber K in the base plate (base element 1) and the central
    The optical see-through channel (viewing device 8) in the cover plate (cover element 4) should be washed around with fresh warm water (temperature control fluid T) as best as possible. Another advantage is the attachment of small edge baffles (side bars 36) in the outer area in order to avoid circulating currents with a long water retention time and the resulting zonal cooling there. The inner baffles (main webs 33) are designed in the form of five angled long and four short straight lines in the base element 1 and in the form of five angled long and five short straight lines in the cover element 4. These guide elements 31 are at an angle of
    72 ° to each other around the center of the plate (central axis Z).
    In Fig. 11, an alternative design of the flow guide in the temperature control chambers 12 and 22 is shown. The cover element 4 is specifically shown in FIG. 11, but the same configuration can also be provided in the base element 1. In this FIG. 11, a meander-shaped temperature control channel 37 delimited by guide walls is formed in the first temperature control chamber 12 and / or the second temperature control chamber 22. This temperature control channel 37 has an inflow section E running radially from the inlet opening 13 in the direction of the incubation chamber K. The first ring section R1 delimited by the inner wall surface 12i directly adjoins this inflow section E. This first ring section R1 is adjoined radially outward by a plurality of ring sections Rn, which lead around the central axis Z and follow one another in a meandering manner. From the outermost ring section Rn, the (cooled) temperature control fluid T then passes back into the first outlet opening 14.This flow channel (temperature control channel 37) is thus laid out in partially annular meanders, whereby the inflowing water should always flow around the central area first in order to avoid heat transport loss to the incubated cells in the incubation chamber K or towards the viewing channel 10
    avoid.
    12 shows a vertical section through the incubator 6, the second intermediate cover 5 and the cover element 4 being shown in dotted lines. This FIG. 12 primarily illustrates the path of the gas G through the incubator 6. In general, FIG. 12 shows the base element 1 with the incubation chamber K, the gas supply chamber 50, the annular humidification chamber 38 and the
    Hot water jacket (second temperature control device 21) shown. The
    Gas supply chamber 50 serves as a gas chamber for preheating the incoming dry gas mixture. This drying gas mixture consists to, preferably about 95%, of air and to, preferably about 5%, of CO ». The first intermediate cover 2 forms a ring-shaped welded, water- and gas-tight cover with a hole (gas outlet opening 58) for gas to pass into the humidification chamber 38. The gas supply opening 39 has a central Luer-Lock connection coupling for a gas supply. This means that gas G can be supplied at around 10 ml per minute at a maximum of 1 bar. A highly absorbent polymer sponge is preferably inserted in the humidification chamber 38. This is designed in a ring shape and enables water to be absorbed of up to 20 ml. The gas G flowing in via the passage opening 52 takes water with it, as a result of which the gas G has an air humidity of up to 98%. The humidification chamber 38 is covered by the cover element 3. This cover element 3 is perforated several times by the gas passage openings 58 and is annular. An atmospheric cavity 61 is formed between the cover element 3 and the second intermediate cover 5. This ensures a barrier-free gas exchange with the incubation chamber K. The cover element 4 has the warm water jacket (first temperature control device 11 including temperature control chamber 12), the sealed viewing device 8 and the drip ribs 60. The gas passage openings 58 have a diameter of approximately 1 mm and are formed in the humidification chamber cover (cover element 3). These are also used to fill with about 20 ml of sterile water. The warm water jacket (temperature control channel 12) in the cover element 4 is used to supply warm water to the viewing device 8. The warm water is supplied by circulation thermostats (to be perfused with 38.5 ° C warm water at 20 ° C room temperature). The viewing channel 10 is closed by disc-shaped viewing panels (viewing windows 91 and 92). As a result, the viewing channel 10 is partially formed by an internally sealed see-through chamber. This is mainly used to avoid the formation of condensation in the optical path. The rising incubation air, which is saturated with water combat, is cooled with the drip ribs 60, as a result of which the water condenses in the lid edge area and the condensed water drips back towards the humidification chamber 38. The second intermediate cover 5 forms a type of disk-shaped faceplate. This is welded in a ring and forms a water-
    and gas-tight barrier to the first temperature chamber 12 and to the viewing channel 10
    Cell observation. After the gas G has reached the incubation chamber K via the atmospheric cavity 61, the gas emerges in a ring at the lid edge of the incubator 6. Specifically, the gas G escapes at about 10 ml per minute through the slight gap between the lid element 4 and the base element 1 the incubator 6. The warm water jacket (temperature chamber 22) in the base element 1 has a warm water supply (inlet opening 23). The temperature control fluid T reaches the temperature control chamber 22 via this inlet opening 23 via a Luer lock coupling. At a room temperature of 22 ° C., the water in this area is still about 37 ° C. warm. That is, the temperature control fluid T has cooled by approximately 1.5 ° C. after flowing through the first temperature control chamber 12. A plurality of (specifically three) annularly closed (concentric) webs 62 are formed on the base element 1. These are about 1 mm high and serve to minimize contact with the ground. This prevents heat loss via the dissipation to the microscope stage. The biological material M at the bottom of the incubation chamber K is formed by living cells. The thickness of the base plate of the base element 1 is approximately 0.6 mm. This makes it suitable for lenses with a magnification of up to 60 times. The section of the bottom element 1 of the incubation chamber K can be surface-treated on the inside. In a preferred embodiment, it is plasma-activated in order to produce a hydrophobic surface for better wettability and cell adhesion. An incubation medium is designated by the reference numeral 63. This incubation medium 63 consists of nutrients and dissolved oxygen / CO ». The nutrient medium (incubation medium 63) supplies the incubated cells with nutrients and the gas exchange with the humidified nutrient gas takes place via its surface. CO »formed by the cells diffuses through the liquid nutrient medium on its surface, while the oxygen required for breathing diffuses from the surface through the nutrient medium to the cells. The incubation chamber K is open at the top and communicates with the atmospheric cavity 61. The humidity of about 98% prevents one
    Evaporation of the incubation medium 63. Referring to FIG. 13, there is a top view of an alternative embodiment of a
    Cover element 4 shown. In the cover element 4 there is a viewing window 9 in the form
    a central lens formed. Specifically, there are four regularly arranged here
    Guide elements 31 are formed in the first temperature control chamber 12. There is one
    full temperature control fluid filling in the cover element 4.
    In Fig. 14, an alternative embodiment of the floor element 1 is shown in perspective. In this case, the underside of the incubator 6 is not continuously disk-shaped. Rather, the underside of the incubator 6 is provided with a clipped-in, frictionally connected glass base 64, which in this case is enclosed by an annular base surface 22b (made of polystyrene) and kept watertight. In this case, the polystyrene base plate in the central chamber area is open in a circle and has retaining or clip bars on the inner edge. In this variant, the chamber floor (instead of 0.6 mm polystyrene) is preferably made of 16 to 20 µm thick, inert borosilicate or quartz glass and is UV-permeable, so that high-resolution microscope images (with up to 100-fold magnification) are possible. In addition, the biological material M can be excited with wavelengths in the UV range in order to also make fluorescence recordings with excitation wavelengths below 360 nm
    enable.
    List of reference symbols
    1 floor element
    2 first intermediate cover 3 cover element
    4 cover element
    5 second intermediate cover
    6 incubator
    7 housing
    8 viewing device
    9 viewing windows
    91 first viewing window
    92 second viewing window
    10 viewing channel
    11 first temperature control device 12 first temperature control chamber
    12i Inner wall surface of the first temperature chamber
    12a outer wall surface of the first temperature chamber 12b bottom surface of the first temperature chamber 12d top surface of the first temperature chamber 13 first inlet opening
    14 first outlet opening
    21 second temperature control device
    22 second temperature chamber
    22i inner wall surface of the second temperature control chamber 22a outer wall surface of the second temperature control chamber 22b bottom surface of the second temperature control chamber 22d top surface of the second temperature control chamber 23 second inlet opening
    24 second outlet opening
    30 wall
    31 guide elements
    32 flow openings
    33 main bridges
    34 first guide section
    35 second guide section
    36 side bars
    37 Temperature control channel
    38 humidification chamber
    39 Gas supply opening
    40 arrangement
    41 Tempering fluid source
    42 Temperature control fluid line
    42a first line section
    42b second line section
    42c third line section
    43 gas source
    44 gas pipe
    45 retaining ring
    46 retaining ring
    47 central opening
    48 outer peripheral surface 49 inner peripheral surface 50 gas supply chamber
    51 cover section
    52 Passage opening
    53 inner peripheral surface 54 outer peripheral surface 55 guide elements
    56 guide recesses 57 central opening
    58 gas openings. 59 drip chambers
    60 drip ribs
    61 atmosphere cavity 62 bars
    63 incubation medium
    64 glass bottom
    M biological material
    K incubation chamber
    T temperature control fluid
    zZ central axis a angle 35 to 34 E inflow section
    R1 first ring section Rn _meander-like successive ring sections G gas
    Innsbruck, April 17, 2019
    权利要求:
    Claims (1)
    [1]
    Claims
    1. Incubator (6) for biological material (M), with
    - a housing (7),
    - an incubation chamber (K) arranged in the housing (7) for a biological material (M),
    - A viewing device (8) arranged in the housing (7) for observing the biological material (M) in the incubation chamber (K), the viewing device (8) having at least one viewing window (9) and one viewing channel (10), and
    - a first temperature control device (11) for the viewing device (8),
    characterized in that the first temperature control device (T1) has a
    A first temperature chamber (12) surrounding the viewing channel (10), a first
    Inlet opening (13) through which a temperature control fluid (T) enters the first
    Temperature chamber (12) can flow, and a first outlet opening (14) over
    which the temperature control fluid (T) can flow out of the first temperature control chamber (12),
    having.
    2. Incubator according to claim 1, characterized in that the housing (7) is essentially cylindrical and has a central axis (Z), this central axis (Z) through the viewing channel (10), the at least one
    Viewing window (9) and the incubation chamber (K) leads.
    3. Incubator according to claim 1 or 2, characterized in that in the housing (7) only one viewing device (8) and only one incubation chamber (K)
    are trained.
    4. Incubator according to one of claims 1 to 3, characterized by a second temperature control device (21) for the incubation chamber (K), the second temperature control device (21) having a second temperature control chamber (22) surrounding the incubation chamber (K), a second inlet opening ( 23), through which a temperature control fluid (T) can flow into the second temperature control chamber (22), and a second outlet opening (24) through which the temperature control fluid (T) exits the second
    Temperature chamber (22) can flow.
    27147
    11.
    Incubator according to at least one of the preceding claims, characterized in that the viewing channel (10) and / or the incubation chamber (K) at least partially from a circular cylinder jacket-shaped or conical jacket-shaped, preferably formed around the central axis (Z)
    Wall (30) are / is limited.
    Incubator according to at least one of the preceding claims, characterized in that the first temperature chamber (12) and / or second
    Tempering chamber (22) are / is ring-shaped around the central axis (Z).
    Incubator according to at least one of the preceding claims, characterized in that the first temperature control chamber (12) and / or the second temperature control chamber (22) are defined by an inner wall surface (12i; 22i), an outer wall surface (12a; 22a), a bottom surface (12b; 22b) and a top surface (12d; 22d) are / is limited.
    Incubator according to claim 7, characterized in that in the first temperature chamber (12) and / or second temperature chamber (22) separate guide elements (31) from the two wall surfaces (12i, 12a; 22i, 22a) for
    Flow line of the inflowing temperature control fluid (T) are arranged.
    Incubator according to Claim 8, characterized in that the guide elements (31) are designed as guide walls which are connected to the bottom surface (12b; 22b) and / or to the top surface (12d; 22d).
    Incubator according to claim 8 or 9, characterized in that the guide elements (31) are spaced apart from the wall surfaces (12i, 12a; 22i, 22a) and between the guide elements (31) and the wall surfaces (12i, 12a; 22i, 22a)
    Through-flow openings (32) are formed. Incubator according to one of claims 8 to 10, characterized in that
    the guide elements (31) at regular intervals from one another and evenly
    are arranged at a distance from a central axis (Z).
    13.
    14th
    15th
    16.
    Incubator according to one of claims 8 to 11, characterized in that the guide elements (31) comprise several, preferably five, main webs (33), the main webs (33) having a first, radially outer, essentially radially aligned, straight guide section (34) ) and a straight, second guide section (35) connected to the first guide section (34) and lying closer to the central axis (Z), the second guide section (35) at an angle (a) between 15 ° and 65 °, preferably
    between 20 ° and 50 °, is aligned with the first guide section (34).
    Incubator according to one of claims 8 to 12, characterized in that the guide elements (31) comprise several, preferably five, side webs (36), the side webs (36) being separate and at a distance from the main webs (33)
    are arranged in the temperature chamber (12; 22).
    Incubator according to claim 7, characterized in that in the first temperature chamber (12) and / or second temperature chamber (22) one of
    Guide walls limited, meander-shaped temperature control channel (37) is formed.
    Incubator according to claim 14, characterized in that the
    Temperature control channel (37)
    - a substantially radially extending inflow section (E),
    - A first ring section (R1), which adjoins the inflow section (E) and is delimited directly by the inner wall surface (12i; 22i) and leading around the central axis (Z)
    - several ring sections (Rn) following one another in a meandering shape, adjoining the first ring section (R1) radially outwards, leading around the central axis (Z)
    includes. Incubator (6) for biological material (M), in particular according to one of the
    Claims 1 to 15, with
    - a multi-part housing (7),
    - An incubation chamber (K) formed by the housing (7) for a biological material (M),
    - A viewing device (8) formed by the housing (7) for observing the biological material (M) in the incubation chamber (K), the viewing device (8) having at least one viewing window (9) and one viewing channel (10),
    - A second temperature control device (21) formed by the housing (7) for the incubation chamber (K), the second temperature control device (21) having a second temperature control chamber (22) surrounding the incubation chamber (K), a second inlet opening (23) via which a Temperature control fluid (T) can flow into the second temperature control chamber (22) and has a second outlet opening (24) through which the temperature control fluid (T) can flow from the second temperature control chamber (22),
    - A humidification chamber (38) formed by the housing (7) and surrounding areas of the incubation chamber (K) and
    - A gas supply opening (39) formed by the housing (7), via which a gas (G) can flow into the incubation chamber (K) via the humidification chamber (38),
    characterized in that the housing (7) completely from at least
    partially transparent, preferably injection molded, plastic
    consists.
    Incubator according to claim 16, characterized in that the housing (7)
    comprises the following five, each one-piece components (1, 2, 3, 4, 5):
    - A base element (1) in which part of the incubation chamber (K), the second inlet opening (23), the second outlet opening (24), the gas supply opening (39) and part of the second temperature chamber (22) are formed,
    - A first intermediate cover (2) which can be connected to the base element (1), preferably with a friction fit, which together with the base element (1) forms the second temperature chamber (22),
    - A perforated cover element (3) arranged on the first intermediate cover (2), which together with the intermediate cover (2) the
    Forms humidification chamber (38),
    19th
    20th
    21st
    22nd
    - A cover element (4) which can be connected to the base element (1), preferably with a friction fit, in which the viewing device (8), the first inlet opening (13), the first outlet opening (14) and part of the first temperature chamber (12) are formed , and
    - One with the cover element (4), preferably frictionally, connectable, second intermediate cover (5), which together with the
    Cover element (4) forms the first temperature chamber (12).
    Incubator according to claim 17, characterized in that a first, preferably lens-shaped, viewing window (91) is formed in the cover element (4) and a second viewing window (92) is formed in the second intermediate cover (5),
    wherein the second viewing window (92) delimits the incubation chamber (K).
    Incubator according to claim 17 or 18, characterized in that the five
    Components (1, 2, 3, 4, 5) consist of the same plastic.
    Incubator according to one of claims 17 to 19, characterized in that
    the plastic is a transparent thermoplastic, preferably polystyrene.
    Arrangement (40) with an incubator (6) according to one of Claims 1 to 20, with
    - a temperature control fluid source (41),
    - A temperature control fluid line (42) which connects the inlet openings (13; 23) and outlet openings (14; 24) with the temperature control fluid source (41),
    - A gas source (43) and
    - A gas line (44) connecting the gas source (43) to the gas supply opening (39).
    Arrangement according to claim 21, characterized in that the tempering fluid line (42) has a first line section (42a) which connects the tempering fluid source (41) to the first inlet opening (13), and a second line section (42b) which connects the first outlet opening (14 )
    connects to the second inlet opening (23).
    having.
    Innsbruck, April 17, 2019
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    同族专利:
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    AT522439B1|2020-11-15|
    WO2020212384A1|2020-10-22|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    DE3924701A1|1989-07-26|1991-01-31|Buehler Edmund Gmbh & Co|Microscope incubator - with transparent lid over petri dish heated by warm air circulation|
    DE4417078A1|1994-05-17|1995-11-23|Fraunhofer Ges Forschung|Chamber for microscopic examination of organic material|
    EP1653269A1|2004-10-28|2006-05-03|Olympus Corporation|Microscope and method of preventing dew condensation on objective lens|
    JP2013158330A|2012-02-08|2013-08-19|Tokai Hit:Kk|Culture apparatus for microscopic observation|
    TW581119U|2001-12-19|2004-03-21|Univ Nat Cheng Kung|Petri dish for microscope|
    AT500473B8|2004-06-28|2007-02-15|Markus Mag Voelp|INCUBATOR|
    DE102013009136A1|2013-05-31|2014-12-04|SunChrom GmbH|lnkubationskammer|
    DE102017104508B3|2017-03-03|2018-03-08|Adolf Kühner Ag|Procedure for moistening an incubator and incubator|WO2021194930A1|2020-03-23|2021-09-30|Nch Corporation|System and method for using a single-serve nutrient spore composition for small scale farm applications|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    ATA50349/2019A|AT522439B1|2019-04-17|2019-04-17|Incubator for biological material|ATA50349/2019A| AT522439B1|2019-04-17|2019-04-17|Incubator for biological material|
    PCT/EP2020/060528| WO2020212384A1|2019-04-17|2020-04-15|Incubator for biological material|
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